[#1, #6, #17, #21, #31] Facility construction and structural installs: prion-capable containment upgrade, optional on-site incinerator build, effluent/wastewater treatment installation, quarantine/clinical isolation retrofit, and initial secure archiving setup.

[#2, #3, #4, #5, #7, #8, #9, #23] Hardware and installed systems: cryogenic freezers and vaulting, backup power/generator/UPS, prion-rated autoclave, corrosion-resistant NaOH immersion tanks, specialized prion instrumentation (RT-QuIC, ultracentrifuge, readers), secure shipping containers and vehicle, Level-A suits inventory, and secure IT hardware.

[#14, #22] Research startup costs: initial breeding colony/startup for animal-models and training/SOP creation and other one-time research program setup.

[#10, #11, #12] Ongoing payroll for scientific staff (PI and senior researchers), technical lab technicians/BSL operators, and security staff including supervision/overtime.

[#7, #18, #29, #14, #20] Reagent kits and assay consumables (RT-QuIC etc.), environmental monitoring (swabs/air/sewage testing), scheduled production/replication runs, ongoing animal husbandry/animal-care in containment, and medical surveillance/occupational health programs.

[#13] Costs for secure D-class custody, housing, medical checks, supervision and associated support for assumed cohort of 10 D-class subjects.

[#32] Multi-year set-aside for major contamination remediation or demolition-level cleanup after significant prion contamination incidents.

[#25] Self-insurance reserve or premiums and allocation for catastrophic liability given limited third-party coverage availability.

[#2, #5, #9, #15, #27] Cryogen (LN2) refills and monitoring, NaOH chemical supply, PPE replacement and consumables, general lab consumables and reagents, decontamination supplies and laundering/disposal.

[#24] Ongoing legal retainer, compliance staffing and liaison/cover-story maintenance as required for high-risk agent research.

[#30] PR/legal rapid-response retainers and public-health liaison budget for containment-breach communications and crisis management.

[#16, #6] Contractor disposal and hazardous-waste handling for NaOH-treated materials, contaminated PPE/animal carcasses, and per-case cremation/controlled disposal contracts when used.

[#19] On-call hazmat/decontamination teams, contractual rapid-response capabilities and smaller contingency reserve for incident response.

[#26] Ongoing staff certification, drills, Level-A suit training, and competency refresher programs.

[#3, #17] Ongoing generator/fuel/testing and routine maintenance plus effluent monitoring/chemicals for wastewater systems.

[#23] Secure IT maintenance, data-logging upkeep, patching and limited air-gapped system servicing.

[#4, #28] Regular calibration, validation and service contracts for autoclaves, RT-QuIC and other safety-critical instruments.

[#8] Recurring courier/shipping per-transport costs and vehicle operation/maintenance for sample transfers and chain-of-custody logistics.

[#31] Ongoing maintenance for secure archival systems and long-term record-keeping for medical/experimental records.