[#30] Contingency/allocation for large-scale eradication or escalation if populations expand (midpoint of provided contingent range).

[#1, #2] Two sealed temperate-forest vivaria construction (primary + backup); midpoint of provided range for site-dependent buildouts.

[#3, #4, #7, #12, #13, #14, #15, #18, #19, #20, #28] HVAC/environmental controllers (two vivaria), generator/UPS capacity, refrigerated freezer units, field vehicles, field equipment, euthanasia equipment & training hardware, CCTV/camera systems, autoclave, cryogenic/freezer units, IT infrastructure, containment breach kits (one-time hardware costs).

[#16] Initial laboratory research project budget for histology, sequencing, imaging and associated project start-up costs.

[#21] One-time permits and application fees (IACUC, environmental permits) at midpoint of estimated range.

[#8, #9, #10, #11, #12] Salaries and benefits for animal-care technicians (3 FTE), part-time/contract veterinarian, 24/7 security coverage (6 guards), and containment/research scientists (assumed 2 senior scientists). Field-recovery personnel costs included within security/staffing estimates.

[#5] Weekly procurement/provisioning of 10 Odocoileus virginianus (midpoint of farm-purchase annual range used as baseline contract).

[#21, #22, #23] Recurring regulatory compliance costs, Class-C amnestic administration and follow-up (estimated annual incident volume), and legal/PR budget for cover stories, liability mitigation and intelligence/PR support.

[#6, #15, #16, #27] Ongoing veterinary care & quarantine services, CCTV storage and video-analysis retention, recurring research budget (sequencing, imaging, assays), and video evidence analysis / archival operations.

[#7, #13, #14, #17, #19, #26, #28] Carcass disposal and refrigerated transport, field-equipment replacement consumables, per-termination consumables, PPE and biohazard consumables, sample consumables for biobanking, replacement/maintenance of plants/substrate per vivarium, and containment kit replenishment.

[#12, #13, #29] Annual vehicle fuel/maintenance for field teams, field equipment replacement/consumables, per-callout operational costs, and public fieldwork/community-relations labor to reduce false reports.

[#20] Ongoing IT operations, backups, secure servers for video/data, and bioinformatics specialist time (recurring operations & specialist salaries portion).

[#24] Property, liability and specialized insurance riders for animal work and biological hazards (midpoint estimate).

[#3, #4, #18, #19] HVAC and environmental controllers energy & maintenance for two vivaria, generator fuel & maintenance, autoclave maintenance, and cryogenic/freezer maintenance.

[#25] Regular training, drills, and contractor-provided exercise costs for containment, animal handling and emergency response.