[#1, #6] Reinforced containment cell upgrade and dedicated secondary chamber for 3 m aggregate (midpoint estimates: $500k + $325k). Includes foundation/structural work, airlock, negative-pressure HVAC modifications, drainage/plumbing.

[#2, #4, #7, #12, #14, #18, #24] Custom plexiglass partition, fitted glove port system, remote manipulators/robotics, emergency neutralization purchase (lasers/thermal cutters), monitoring installation, hardened transport vehicle & containment hardware, and initial secure IT setup (sum of midpoint estimates).

[#8, #9, #10] Continuous on-site security detail, supervisors, research personnel salaries (senior scientist + techs), and medical/psychiatric staffing; includes benefits and overtime.

[#23] Optional/selected multi-year neutralization R&D allocation (advanced experiments, pilot neutralization trials); treated as a recurring/project allocation in planning.

[#20] Recommended 15–25% reserve of baseline operating costs for emergent behaviors, breach repairs, or rapid facility expansion (annual allocation).

[#10, #21] Non-salary research costs: histology, sequencing, microscopy consumables, controlled experiments, and specialized pathology/diagnostic reagents (research program consumables separate from staff wages).

[#19] Permits, legal counsel, compliance, and cover-story / public-relations expenditures.

[#3, #5, #13, #16] Polymer gel MCN119 supply (daily refill), replacement disposable liners/gloves, routine PPE replacement (hazmat suits, cut-resistant gear), and specialized cleaning solvents/consumables.

[#12, #14, #15, #25] Ongoing maintenance/calibration for emergency neutralization equipment, storage & monitoring recurring costs, environmental control/HVAC/dehumidifier energy and upkeep, and amortized replacement of partitions/glove-port seals/robotics.

[#11] Incineration/autoclave/chemical neutralization runs, contracted hazardous-waste transport and rated removal for biohazardous animate tissue.

[#18] Annual transfer operations for secure transfers: crew, fuel, sealed-container use and ops (vehicle purchase accounted one-time).

[#17] Regular staff training, breach drills, glove-refill procedures, manipulator ops, and psychiatric de-escalation simulations.

[#22] Ongoing therapy, enrichment, medications and monitoring for SCP-4597 to reduce self-harm and associated containment risk.

[#24] Secure data storage retention, encrypted backups, and recurring IT costs (initial setup in one-time equipment).