[#25] Program-level duplicate-site premium to provision a second containment cell (and many duplicated capital items) — applied as an 80% premium on single-site capital to reflect partial centralization efficiencies.

[#2, #3, #4, #5, #6, #7, #8, #9, #10, #17, #18, #20] HVAC install, dedicated greenhouse hardware, redundant outgoing filtration, EM coils, EM-shielded CCTV, scram-system engineering + initial chemical inventory, hazardous-chemical storage hardware, backup freezer purchase, autoclave purchase, environmental sensors install, UPS/generator, secure-transport vehicle retrofits (single-site baseline equipment costs).

[#15] Specialized research equipment (microscopes, electrophysiology rigs, sequencing/mass-spec) initial purchase and lab buildout (single-site baseline).

[#1] Single-cell containment construction/retrofit (BSL-3/4-grade sealed habitat, airlocks, reinforced walls, observation deck).

[#26] Contingency / reserve allocation for unforeseen engineering, legal, or incident expenses (one-time program allocation).

[#30] Decommissioning / sanitization reserve (one-time when applicable).

[#22] One-time legal/regulatory setup, permits and initial compliance paperwork.

[#12, #13, #14] Program-level personnel salaries: containment technicians (6), security guards (10), research staff (1 senior + 2 assistants).

[#15, #17, #27] Research operations, equipment maintenance & consumables, environmental monitoring / periodic PCR testing, and ongoing experimental campaigns to study electrical activity and behavior.

[#21] On-call hazmat rapid-response readiness, drills, medical readiness and remediation training and exercises.

[#2, #4, #5, #18, #19, #10] HVAC power & maintenance, filter replacements, EM coils power & maintenance, generator/UPS fuel & testing, scheduled maintenance contracts, autoclave maintenance.

[#3, #11, #16, #24, #6] Greenhouse consumables & electricity, PPE and decontamination supplies, sampling/analytical consumables, consumable plant-matter replacement, CCTV battery replacements/monitoring.

[#22, #26] Ongoing legal/regulatory counsel, permit renewals, and cover-story/OPSEC budget; includes recurring insurance premiums.

[#7] Recurring chemical inventory, licensing, hazardous-material training and compliance for scram neutralization system (replacement inventory / compliance costs).

[#28] Annual amortized replacement reserve for HVAC, EM coils, freezers, pumps, cameras and other capital equipment.

[#8] Periodic inspections, leak detection, certification, and compliance-related operational costs for hazardous-chemical storage and safety systems.

[#29] Staff rotation, counseling, extra staffing/operational safety overhead to mitigate human-factors and burnout.

[#20] Secure transport operations, specialized containment boxes, trained transport crews and chain-of-custody logistics (annual operations).

[#23] Staff training, certifications, vaccinations, and medical surveillance programs.

[#9] LN2 / ultra-low freezer supply, monitoring, alarm servicing and maintenance for cryogenic spore backups.

[#10] Biohazard waste treatment and occasional contract incineration / manifesting costs (if used) and validation costs.