[#1, #3, #7] Structural and room-level work: biocontainment retrofit (negative-pressure room, anterooms, HEPA), freezer room modifications and secondary containment racks, and incinerator installation.

[#1, #2, #4, #9, #10, #22] Major hardware purchases and installed systems: −80°C cryogenic freezers and monitoring, redundant generator/UPS/ATS, access-control/CCTV/tamper sensors, autoclave purchase, secure transport cases and refrigerated vehicle, initial secure-data hardware/setup.

[#13] Research-centric capital: bench instruments or core-access equivalent (GC‑MS/LC‑MS, sequencer access, EM/HPLC time, centrifuges) and initial lab buildout for experimental work.

[#16] Initial SOP development, tabletop and live drills, and initial training sessions.

[#19] Initial deep background checks for core team (10-person estimate).

[#23] Initial specialized chemical agents/reagent suite for dissolving saccharide shells and neutralization for analysis.

[#9] Initial stock of chemical disinfectants, spill kits and decon consumables.

[#5] Salaries and benefits for containment biologists, lab techs, security officers, entomological consultant and minimal support staff (includes overhead/shift coverage).

[#24] Annual allocation to a contingency fund/reserve to finance large-scale quarantine, mass incineration, emergency evacuations and cross-jurisdiction coordination in catastrophic scenarios.

[#10, #11, #25] Field deployment and transport costs: on-call rapid-response hazmat team maintenance/deployments, per-deployment vehicle/crew costs, and transport/disposal/recall handling for contaminated commercial products.

[#13, #26, #21, #17] Recurring research service contracts/consumables, ongoing entomological/ecological surveillance, facility pest-containment monitoring/traps and medical surveillance/employee health monitoring.

[#9, #15, #20, #14, #8, #23] Recurring decontamination consumables, PPE replenishment, consumable biological material procurement, sample-storage/quarantine costs, contracted biohazard disposal, reagent replenishment.

[#6] Annual budget for D‑Class/expendable test subjects, housing, procurement and legal overhead (where applicable).

[#2, #4, #7, #22] Generator/LN2 service and fuel, security/CCTV monitoring contracts and maintenance, incinerator operation & maintenance and hazardous-ash disposal service, secure-data hosting/maintenance.

[#12] Annualized average for routine per-incident containment/neutralization (seal site, recover specimens, incinerate remains, amnestics/cover) averaged across expected low-frequency events.

[#18] Ongoing budget for minor legal/PR/cover-story expenses, witness management and low-level incident legal handling.

[#16] Annual refresher training, drills and SOP updates.

[#19] Periodic background rechecks and security-clearance renewals.