[#2, #3, #5, #7, #8, #12, #15, #16, #20, #22, #24, #27, #28, #30] One-time equipment and capital: legacy heavy-containment option (osmium/copper/platinum — prohibitive, large line-item), UPS/subpanel and electrical hardware, N2O venting/scavenging system, shock-pulse hardware, surveillance systems, PPE procurement, veterinary/surgical plates & associated kit, spare module inventory and electronics bench, locks/keys/tamper hardware, data-logging/archival hardware, transport case, contingency/liability reserve, long-term archive setup, and decommissioning reserve.

[#2] Legacy/procedural heavy containment (3 m osmium walls, copper alloy lining, platinum tiling) — order-of-magnitude conservative lower bound; effectively prohibitive and likely infeasible.

[#27] Contingency reserve for rapid rebuild, emergency procurement, or major containment upgrades.

[#30] Estimated reserved funds for secured disposal, remediation and legal/ethical reviews if termination/decommissioning is required.

[#17] Laboratory buildout and core research instruments (DNA sequencer access or in‑house sequencer, microscopes, oscilloscopes, EMG rigs and tissue-handling gear) to support genomic and module-interface study.

[#1, #14] Construction/modification of a reinforced animal containment cell (4 outlets, negative-pressure seals, pass-throughs, camera mounts) and a decontamination station (airlock/showers/sterilizers).

[#10] On-call Mobile Task Force readiness allocation (training, equipment, vehicle maintenance, exercises and availability costs).

[#9, #11] Security staffing (9 FTE guards, benefits and overtime) plus dedicated research staff (1 senior scientist, 2 technicians, 1 containment engineer) including benefits.

[#26] Legal, cover-story maintenance, information-security operations, PR and compensation for local stakeholders as needed to maintain secrecy.

[#13, #16, #17, #18, #21, #22] Medical monitoring/post-exposure exams, annual calibration/repair of spare modules, sequencing/assay supplies, genomic monitoring and diagnostics, psychological monitoring and staff-rotation programs, and IT/storage maintenance for research data.

[#6, #12, #19, #25] Nitrous oxide consumables & compliance costs (bulk or cylinders), PPE replacements and retraining consumables, small consumables/repair items, and hazardous/bioelectronic waste disposal fees.

[#29] Insurance/financial overhead and administrative support allocations.

[#23] Periodic multi-unit emergency drills, evacuation planning and exercises with associated costs.

[#24] Per-transfer logistics, planned secure transfers and vehicle/handler costs (assumes occasional transfers per year).

[#8] Camera, sensor and alarm maintenance, storage and redundancy upkeep.

[#3] General site and containment cell maintenance, periodic certification of seals and structural inspections.

[#4] Electrical energy to power specimens (estimated ~40,000 kWh/yr at $0.10–$0.15/kWh).

[#3] UPS/backup maintenance, periodic power-electronics testing and generator transfer checks.

[#28] Ongoing curation, storage and environmental control for archived containment iterations and artifacts.

[#7] Maintenance, recertification and testing of timed-current control hardware and safety interlocks.

[#20] Audits, lock replacements and tamper-evidence logistics for hatch/key systems.