[#1, #2, #4, #6, #16, #22, #24] Construction/fitout of 20 isolated bio-containment chambers, wing-level HVAC/negative-pressure systems, structural reinforcement/sacrificial bays, central cold-room installation, wastewater/effluent containment upgrades, access/control route modifications and communications isolation installations.

[#3, #5, #7, #8, #13, #14, #15] Backup generator/UPS, on-site incinerator/grinder, lighting fixtures, automated chamber hardware (cameras/sensors), transport crates and secure vehicle, secure servers, autoclave and initial PPE stock.

[#12] Dedicated one-time contingency/reserve for Stage B countermeasures, emergency equipment procurement and initial emergency fund capitalization.

[#11, #20, #21, #19] Demoralization R&D setup and lab buildout, capital laboratory equipment (microscopy, sequencing, GC-MS/LC-MS), initial training/clearance processing one-time costs and initial permitting/compliance setup.

[#9] Salaries, benefits and overhead for Level-3 researchers, containment technicians, security guards and biohazard disposal specialists (annualized total; mid-range estimate).

[#10] Additional FTEs or overtime to cover 4×/day morale checks per instance and associated increased shift/operational labor time.

[#8, #11, #14, #20] Ongoing software license/support and ML model upkeep, demoralization/behavioral R&D operational costs, cloud storage/analysis retention, reagents and laboratory consumables for experiments and monitoring.

[#5, #13] Contracted hazardous disposal per-ton recurring costs and recurring vehicle/driver/security operations for specimen transport.

[#7, #15, #25] Replacement fluorescent bulbs/lighting maintenance, PPE/consumables replacement and miscellaneous operational consumables (cleaning supplies, tooling, small parts).

[#23] Retainer for emergency heavy-equipment access (forklifts, vacuum trucks, grinders) to respond to rapid biomass expansion events.

[#18] Baseline legal, cover-story/disinformation operations and consulting to manage procurement records and external inquiries (does not include episodic amnestic incident costs).

[#6, #16, #19, #24] Ongoing HVAC/cold-room energy & maintenance, wastewater monitoring/treatment, regulatory reporting/permitting renewals and communications/isolation system maintenance.

[#17] Annual occupational health, allergy testing, vaccinations and routine medical surveillance for ~30 staff.

[#21] Annual refresher courses, emergency drills and ongoing training; initial one-time clearance costs are captured in one-time setup.