[#12, #13, #20] Initial analytical/toxicology investigative budget, partial custom standard synthesis and reagents, initial R&D/mitigation program seed funding, and LIMS/database setup for sample/chain-of-custody records.

[#1, #2, #15] Sealed containment room construction/retrofitting, HVAC installation/ducting and controls, and initial security/keycard/CCTV install (structural and installation work).

[#3, #5, #7, #8, #14, #22] Containment furniture and primary equipment (cages, incubators, microscopes, workbenches), suit decontamination station, refrigerators/freezers, on-site autoclave (if chosen), initial monitoring install (sticky/CO2 traps installation), and sealed transport containers.

[#19] Institutional biosafety/IACUC/permit setup and initial compliance consulting.

[#10] Initial training program setup, bloodborne-pathogen training and occupational health program startup costs.

[#11] Initial purchase of naloxone kits and on-site emergency medical kit + initial training costs.

[#23] Catastrophic containment-failure reserve for major public-health remediation, legal liability, and large-scale cleanup/remediation.

[#9] Salaries for 1 senior entomologist and 2 technicians plus ~30% fringe/benefits (annualized).

[#16] Annual contribution to rapid-response contingency fund for eradication/fumigation/incident remediation (recommended reserve level).

[#24] Administrative overhead, proportional rent/utilities and procurement/administrative support (estimated at ~25% of direct ops).

[#10, #12, #13, #14] Ongoing toxicology/LC-MS testing, occupational health surveillance and testing, ongoing R&D program funding, and monitoring consumables (eDNA swabs, sticky/CO2 trap consumables).

[#17, #22] Field response team deployments / covert extermination operational costs and per-transfer courier/secure transport service costs.

[#6] Consumable blood supply for feeding colonies (example scale: ~10,000 insects; annual animal blood costs estimated in notes).

[#2, #15, #20] HVAC/environmental control operating costs, security/CCTV monitoring and badge systems maintenance, and LIMS/freezer power & maintenance.

[#18] PR/legal retainer and liaison costs for cover stories, public health liaison, and limited lobbying/PR activity.

[#19] Annual permit renewals, inspections, institutional compliance fees and liability insurance premiums (ongoing).

[#4, #5, #21] PPE replacement and visitor stock, insecticide consumables for decon, and routine entomology consumables (harborages, adhesives, slides).

[#7] Consumables for blood handling: reagents, syringes, sterile tubing, membranes and related monthly consumables.

[#8] Routine biohazardous waste handling/disposal (contractor disposal or on-site autoclave maintenance/consumables).

[#11] Ongoing naloxone kit replacement, training refreshers, and emergency medical contract standby budget.