[#1, #21] Large-scale aquatic containment construction (tank/quarry renovation, excavation, concrete, access platforms, immediate security/perimeter structures and initial redundant perimeter/sacrificial panels). Estimate assumes mid-range new-build cost plus initial perimeter work.

[#15] BSL-3/4 lab upgrades, pathology and molecular analysis equipment (sequencers, microscopes, cryostorage, negative-pressure rooms) and secure sample handling spaces.

[#2, #3, #4, #5, #6, #7, #8, #9, #10, #12, #13] Submerged steel chamber fabrication & installation; remote-actuated restraints; initial rods/spacing panels; pumps and circulation hardware; backup power hardware (generators/ATS); filtration/sterilization equipment; initial corrosion coatings; structural/surface sensors; video/surveillance hardware; one-time specialized response equipment and advanced immobilization unit procurement.

[#18] Seed emergency/rapid-response reserve (liquid fund for immediate containment breaches: helicopter time, emergency repairs, rapid lockdown, local coordination).

[#20] One-time reserve/escrow for liability and major reconstruction exposure (seed for insurance/risk capital).

[#27] Contingency for controlled demolition, hazardous waste removal and environmental remediation if site must be destroyed or abandoned.

[#28] One-time scientific contingency for unexpected properties requiring specialised equipment, outside expertise, or emergency relocations.

[#14] One-time R&D and prototyping budget for anti-stitch surfaces, active deterrents, materials testing and iterative prototyping.

[#16] Cryostorage, forensic processing setup, sterile kits and initial reagent stock for long-term tissue/DNA banks and autopsy capacity.

[#26] Initial hardened archival infrastructure and offline/backups for logs, video and chain-of-custody records.

[#11, #12] 24/7 containment staffing (guards/operators, surveillance operators, maintenance techs) plus specialized response/recovery team salaries, benefits and hazard pay.

[#14, #15, #28, #16] Continued materials testing, prototype iterations, BSL lab operational costs (consumables, sequencing, pathology), and recurring scientific contingency spend for unexpected findings.

[#19] Legal counsel, PR/cover operations, witness management and covert relocations; variable with incident frequency.

[#4, #8, #22, #21, #9] Ongoing structural maintenance, corrosion protection recoating/drydock, inspection cycles, sacrificial panel replacement stock and sensor calibration/maintenance.

[#20] Annual premiums or reserve replenishment for liability/risk transfers.

[#7, #13, #16, #25, #29] Filtration consumables and treatment chemicals, immobilization consumables (cartridges/spares), sample consumables and sequencing runs, baseline decontamination consumables, routine spares (gaskets, seals, fasteners).

[#18] Annual replenishment to keep rapid-response fund liquid and at target levels after use.

[#5, #6] Electricity for continuous high-power circulation pumps (20–120 kW load) plus generator fuel and generator maintenance amortized per year.

[#24] Recurring exercises, certification, simulator training for containment, medics, technical staff and emergency responders.

[#23] Heavy-lift rentals, crane time, flatbeds, armored transport and related fuel/maintenance for equipment moves and recoveries.

[#17] Incineration, chemical neutralization, compliant disposal and transport of biomass and casualties (routine baseline; spikes during incidents).

[#10, #26] Secure on-site and off-site storage, bandwidth and archival admin for 24/7 high-resolution surveillance and experiment records.