[#2, #12, #17, #18, #22, #28] Initial substrate/bedding fill for vivaria, initial specialized training for staff, initial research equipment procurement (bioacoustics/analytical instruments) and secure-digitization/redaction workflow for artwork, quarantine setup for new accessions, and allowance reflecting modest program-scale setup as described in guidance.

[#1, #5, #7, #8, #9, #16, #19, #23] Vivaria (10 units), incubator/chamber hardware, small off-grid solar + LED lighting per room, ventilation/HEPA & insect-barrier hardware, initial PPE stock, initial decontamination supplies, ultrasonic microphones/recorders and analysis hardware, air-gapped/data-storage hardware, and initial sensors.

[#4, #6, #15, #18, #24] Room-level HVAC/climate-control installation and modulation, acoustic/ultrasonic dampening and sealing, electronic locks/CCTV installation, secure archive/safe installation and basic generator/UPS infrastructure for redundancy.

[#22] One-time quarantine space/equipment setup per new accession (included here as an explicit one-time line item from note #22).

[#13, #15, #26] Two full-time husbandry/technician positions (salary + benefits), minimal on-site security/guarding costs (part-time/full-time equivalent), and one memetic/archive safety officer (salary + benefits).

[#11, #12, #14, #16, #17, #18, #19, #23, #27] Ongoing occupational medical surveillance and baseline psychiatric support, refresher training, veterinary retainer/consultations, ultrasonic monitoring software/licenses, research consumables and sample analysis, secure-archive oversight and controlled-digitization oversight, data-security maintenance for air-gapped systems, sensor calibration, and psychological support services.

[#20] Annual legal/PR retainer and readiness budget to manage potential disclosure or public incidents (baseline retainer; large incidents handled via scenarios/contingency).

[#21] Facility and liability insurance premiums for occupational exposures/property/litigation for a small specialized program.

[#2, #3, #8, #9, #29] Annual substrate replenishment, weekly fresh-food provisioning for 10 vivaria, recurring PPE/disposables, decontamination consumables, and miscellaneous small consumables and replacements.

[#11, #20, #25] Annual allocation to a reserve fund to cover episodic hospitalizations, emergency cleanup/remediation, PR/legal contingency spending and rapid contractor mobilization.

[#10, #22] Hazardous/regulated waste disposal contracts and routine transport/shipping/quarantine costs for specimen movements.

[#4, #5, #7, #15, #23, #24] HVAC/incubator calibration and maintenance, solar system maintenance, ventilation filter replacements, CCTV/locks maintenance, sensor calibration/replacement, and generator/UPS maintenance.